Phenazine virulence factor binding to extracellular DNA is important for Pseudomonas aeruginosa biofilm formation.

ABSTRACT: Bacterial resistance to conventional antibiotics necessitates the identification of novel leads for infection control. Interference with extracellular phenomena, such as quorum sensing, extracellular DNA integrity and redox active metabolite release, represents a new frontier to control human pathogens such as Pseudomonas aeruginosa and hence reduce mortality. Here we reveal that the extracellular redox active virulence factor pyocyanin produced by P. aeruginosa binds directly to the deoxyribose-phosphate backbone of DNA and intercalates with DNA nitrogenous base pair regions. Binding results in local perturbations of the DNA double helix structure and enhanced electron transfer along the nucleic acid polymer. Pyocyanin binding to DNA also increases DNA solution viscosity. In contrast, ant

SUBMITTER: Das T 

PROVIDER: S-EPMC4323658 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature