Localization of complement factor H gene expression and protein distribution in the mouse outer retina.

ABSTRACT:

Purpose

To determine the localization of complement factor H (Cfh) mRNA and its protein in the mouse outer retina.

Methods

Quantitative real-time PCR (qPCR) was used to determine the expression of Cfh and Cfh-related (Cfhr) transcripts in the RPE/choroid. In situ hybridization (ISH) was performed using the novel RNAscope 2.0 FFPE assay to localize the expression of Cfh mRNA in the mouse outer retina. Immunohistochemistry (IHC) was used to localize Cfh protein expression, and western blots were used to characterize CFH antibodies used for IHC.

Results

Cfh and Cfhr2 transcripts were detected in the mouse RPE/choroid using qPCR, while Cfhr1, Cfhr3, and Cfhrc (Gm4788) were not detected. ISH showed abundant Cfh mRNA in the RPE of all mouse strains (C57BL/6, BALB/c, 129/Sv)

SUBMITTER: Smit-McBride Z 

PROVIDER: S-EPMC4323684 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature