Barcoding of live human peripheral blood mononuclear cells for multiplexed mass cytometry.

ABSTRACT: Mass cytometry is developing as a means of multiparametric single-cell analysis. In this study, we present an approach to barcoding separate live human PBMC samples for combined preparation and acquisition on a cytometry by time of flight instrument. Using six different anti-CD45 Ab conjugates labeled with Pd104, Pd106, Pd108, Pd110, In113, and In115, respectively, we barcoded up to 20 samples with unique combinations of exactly three different CD45 Ab tags. Cell events carrying more than or less than three different tags were excluded from analyses during Boolean data deconvolution, allowing for precise sample assignment and the electronic removal of cell aggregates. Data from barcoded samples matched data from corresponding individually stained and acquired samples, at cell event recover

SUBMITTER: Mei HE 

PROVIDER: S-EPMC4323739 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature