Genome-wide identification of Bacillus subtilis Zur-binding sites associated with a Zur box expands its known regulatory network.

ABSTRACT:

Background

The Bacillus subtilis Zur transcription factor recognizes a specific DNA motif, the Zur box, to repress expression of genes in response to zinc availability. Although several Zur-regulated genes are well characterized, a genome-wide mapping of Zur-binding sites is needed to define further the set of genes directly regulated by this protein.

Results

Using chromatin immunoprecipitation coupled with hybridization to DNA tiling arrays (ChIP-on-chip), we reported the identification of 80 inter- and intragenic chromosomal sites bound by Zur. Seven Zur-binding regions constitute the Zur primary regulon while 35 newly identified targets were associated with a predicted Zur box. Using transcriptional fusions an intragenic Zur box was showed to promote a full Zur-mediated r

SUBMITTER: Prestel E 

PROVIDER: S-EPMC4324032 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature