Role of extracytoplasmic function sigma factors in biofilm formation of Porphyromonas gingivalis.

ABSTRACT:

Background

Porphyromonas gingivalis has been implicated as a major pathogen in the development and progression of chronic periodontitis. P. gingivalis biofilm formation in the subgingival crevice plays an important role in the ability of the bacteria to tolerate stress signals outside the cytoplasmic membrane. Some bacteria use a distinct subfamily of sigma factors to regulate their extracytoplasmic functions (the ECF subfamily). The objective of this study was to determine if P. gingivalis ECF sigma factors affect P. gingivalis biofilm formation.

Methods

To elucidate the role of ECF sigma factors in P. gingivalis, chromosomal mutants carrying a disruption of each ECF sigma factor-encoding gene were constructed. Bacterial growth curves were measured by determining the turbid

SUBMITTER: Onozawa S 

PROVIDER: S-EPMC4324044 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature