Dexmedetomidine and fentanyl exhibit temperature dependent effects on human respiratory cilia.

ABSTRACT:

Background

Dexmedetomidine (dex) is commonly used in intensive care due to its effective sedation and analgesia with few adverse effects and minimal respiratory depression. However, we recently observed that exposing mouse epithelial respiratory cells to dex decreased ciliary beat frequency (CBF), suggesting dex may pose pulmonary risk.

Objective

The purpose of this study is to determine the effects of dex at clinically relevant doses on CBF in human respiratory epithelia.

Methods

Human nasal epithelial cilia were obtained from the inferior nasal turbinate with a rhinoprobe and placed in culture medium at 15°C and 37°C. At 5 and 30 min, video-microscopy was used to assess CBF, either without (control) or with different concentrations (1, 5, and 10 nM) of dex, fentanyl

SUBMITTER: Welchering N 

PROVIDER: S-EPMC4324059 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature