Genetic manipulation of the Toxoplasma gondii genome by fosmid recombineering.

ABSTRACT:

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Apicomplexa are obligate intracellular parasites that cause important diseases in humans and animals. Manipulating the pathogen genome is the most direct way to understand the functions of specific genes in parasite development and pathogenesis. In Toxoplasma gondii, nonhomologous recombination is typically highly favored over homologous recombination, a process required for precise gene targeting. Several approaches, including the use of targeting vectors that feature large flanks to drive site-specific recombination, have been developed to overcome this problem. We have generated a new large-insert repository of T. gondii genomic DNA that is arrayed and sequenced and covers 95% of all of the parasite's genes. Clones from this fosmid library are maintained at single cop

SUBMITTER: Vinayak S 

PROVIDER: S-EPMC4324243 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature