Possibilities and limitations of current technologies for quantification of biological extracellular vesicles and synthetic mimics.

ABSTRACT: Nano-sized extracelullar vesicles (EVs) released by various cell types play important roles in a plethora of (patho)physiological processes and are increasingly recognized as biomarkers for disease. In addition, engineered EV and EV-inspired liposomes hold great potential as drug delivery systems. Major technologies developed for high-throughput analysis of individual EV include nanoparticle tracking analysis (NTA), tunable resistive pulse sensing (tRPS) and high-resolution flow cytometry (hFC). Currently, there is a need for comparative studies on the available technologies to improve standardization of vesicle analysis in diagnostic or therapeutic settings. We investigated the possibilities, limitations and comparability of NTA, tRPS and hFC for analysis of tumor cell-derived EVs and syn

SUBMITTER: Maas SL 

PROVIDER: S-EPMC4324667 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature