Quantification of HDL particle concentration by calibrated ion mobility analysis.

ABSTRACT:

Background

It is critical to develop new metrics to determine whether HDL is cardioprotective in humans. One promising approach is HDL particle concentration (HDL-P), the size and concentration of HDL in plasma. However, the 2 methods currently used to determine HDL-P yield concentrations that differ >5-fold. We therefore developed and validated an improved approach to quantify HDL-P, termed calibrated ion mobility analysis (calibrated IMA).

Methods

HDL was isolated from plasma by ultracentrifugation, introduced into the gas phase with electrospray ionization, separated by size, and quantified by particle counting. We used a calibration curve constructed with purified proteins to correct for the ionization efficiency of HDL particles.

Results

The concentrations of gol

SUBMITTER: Hutchins PM 

PROVIDER: S-EPMC4324763 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature