TOX3 is expressed in mammary ER(+) epithelial cells and regulates ER target genes in luminal breast cancer.

ABSTRACT:

Background

A breast cancer susceptibility locus has been mapped to the gene encoding TOX3. Little is known regarding the expression pattern or biological role of TOX3 in breast cancer or in the mammary gland. Here we analyzed TOX3 expression in murine and human mammary glands and in molecular subtypes of breast cancer, and assessed its ability to alter the biology of breast cancer cells.

Methods

We used a cell sorting strategy, followed by quantitative real-time PCR, to study TOX3 gene expression in the mouse mammary gland. To study the expression of this nuclear protein in human mammary glands and breast tumors, we generated a rabbit monoclonal antibody specific for human TOX3. In vitro studies were performed on MCF7, BT474 and MDA-MB-231 cell lines to study the effects of

SUBMITTER: Seksenyan A 

PROVIDER: S-EPMC4324787 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature