Genome instability mediates the loss of key traits by Acinetobacter baylyi ADP1 during laboratory evolution.

ABSTRACT: Acinetobacter baylyi ADP1 has the potential to be a versatile bacterial host for synthetic biology because it is naturally transformable. To examine the genetic reliability of this desirable trait and to understand the potential stability of other engineered capabilities, we propagated ADP1 for 1,000 generations of growth in rich nutrient broth and analyzed the genetic changes that evolved by whole-genome sequencing. Substantially reduced transformability and increased cellular aggregation evolved during the experiment. New insertions of IS1236 transposable elements and IS1236-mediated deletions led to these phenotypes in most cases and were common overall among the selected mutations. We also observed a 49-kb deletion of a prophage region that removed an integration site, which has been u

SUBMITTER: Renda BA 

PROVIDER: S-EPMC4325111 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature