454 screening of individual MHC variation in an endemic island passerine.

ABSTRACT: Genes of the major histocompatibility complex (MHC) code for receptors that are central to the adaptive immune response of vertebrates. These genes are therefore important genetic markers with which to study adaptive genetic variation in the wild. Next-generation sequencing (NGS) has increasingly been used in the last decade to genotype the MHC. However, NGS methods are highly prone to sequencing errors, and although several methodologies have been proposed to deal with this, until recently there have been no standard guidelines for the validation of putative MHC alleles. In this study, we used the 454 NGS platform to screen MHC class I exon 3 variation in a population of the island endemic Berthelot's pipit (Anthus berthelotii). We were able to characterise MHC genotypes across 309 indivi

SUBMITTER: Gonzalez-Quevedo C 

PROVIDER: S-EPMC4325181 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature