Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera.

ABSTRACT: AIM:To identify the presence of various bone morphogenetic proteins (BMPs) and their receptors in normal sclera of human, rat and guinea pigs, and to determine whether their expression changed with form-deprivation myopia (FDM) in guinea pig sclera. METHODS:The expression of BMPs and BMP receptors were detected using reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescence. Two-week-old guinea pigs were monocularly form-deprived with a translucent lens. After fourteen days induction of FDM, total RNA was isolated and subjected to RT-PCR to examine the changes of BMPs and BMP receptors in tissues from the posterior sclera. Western blotting analysis was used to investigate their changes in protein levels. RESULTS:Human sclera expressed mRNAs for BMP-2, -4, -5, -7, -RIA

SUBMITTER: Wang Q 

PROVIDER: S-EPMC4325239 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature