Duct cells contribute to regeneration of endocrine and acinar cells following pancreatic damage in adult mice.

ABSTRACT:

Background & aims

There have been conflicting results on a cell of origin in pancreatic regeneration. These discrepancies predominantly stem from lack of specific markers for the pancreatic precursors/stem cells, as well as differences in the targeted cells and severity of tissue injury in the experimental models so far proposed. We attempted to create a model that used diphtheria toxin receptor (DTR) to ablate specific cell populations, control the extent of injury, and avoid induction of the inflammatory response.

Methods

To target specific types of pancreatic cells, we crossed R26DTR or R26DTR/lacZ mice with transgenic mice that express the Cre recombinase in the pancreas, under control of the Pdx1 (global pancreatic) or elastase (acinar-specific) promoters.

Results

SUBMITTER: Criscimanna A 

PROVIDER: S-EPMC4326039 | biostudies-literature | 2011 Oct

REPOSITORIES: biostudies-literature