Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing.

ABSTRACT:

Aim

To identify the genes induced and regulated by the MYC protein in generating tumors from liver stem cells.

Methods

In this study, we have used an immortal porcine liver stem cell line, PICM-19, to study the role of c-MYC in hepatocarcinogenesis. PICM-19 cells were converted into cancer cells (PICM-19-CSCs) by overexpressing human MYC. To identify MYC-driven differential gene expression, transcriptome sequencing was carried out by RNA sequencing, and genes identified by this method were validated using real-time PCR. In vivo tumorigenicity studies were then conducted by injecting PICM-19-CSCs into the flanks of immunodeficient mice.

Results

Our results showed that MYC-overexpressing PICM-19 stem cells formed tumors in immunodeficient mice demonstrating that a singl

SUBMITTER: Aravalli RN 

PROVIDER: S-EPMC4326136 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature