Refactoring the silent spectinabilin gene cluster using a plug-and-play scaffold.

ABSTRACT: Natural products (secondary metabolites) are a rich source of compounds with important biological activities. Eliciting pathway expression is always challenging but extremely important in natural product discovery because an individual pathway is tightly controlled through a unique regulation mechanism and hence often remains silent under the routine culturing conditions. To overcome the drawbacks of the traditional approaches that lack general applicability, we developed a simple synthetic biology approach that decouples pathway expression from complex native regulations. Briefly, the entire silent biosynthetic pathway is refactored using a plug-and-play scaffold and a set of heterologous promoters that are functional in a heterologous host under the target culturing condition. Using this

SUBMITTER: Shao Z 

PROVIDER: S-EPMC4326240 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature