A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes.

ABSTRACT: Diagnostic methods for detecting and quantifying HIV RNA have been improving, but efficient methods for point-of-care analysis are still needed, particularly for applications in resource-limited settings. Detection based on reverse-transcription loop-mediated isothermal amplification (RT-LAMP) is particularly useful for this, because when combined with fluorescence-based DNA detection, RT-LAMP can be implemented with minimal equipment and expense. Assays have been developed to detect HIV RNA with RT-LAMP, but existing methods detect only a limited subset of HIV subtypes. Here we report a bioinformatic study to develop optimized primers, followed by empirical testing of 44 new primer designs. One primer set (ACeIN-26), targeting the HIV integrase coding region, consistently detected subtype

SUBMITTER: Ocwieja KE 

PROVIDER: S-EPMC4326360 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature