MiR-503 represses human cell proliferation and directly targets the oncogene DDHD2 by non-canonical target pairing.

ABSTRACT:

Background

The pathways regulating the transition of mammalian cells from quiescence to proliferation are mediated by multiple miRNAs. Despite significant improvements in our understanding of miRNA targeting, the majority of miRNA regulatory networks are still largely unknown and require experimental validation.

Results

Here we identified miR-503, miR-103, and miR-494 as negative regulators of proliferation in primary human cells. We experimentally determined their genome wide target profiles using RNA-induced silencing complex (RISC) immunoprecipitations and gene expression profiling. Analysis of the genome wide target profiles revealed evidence of extensive regulation of gene expression through non-canonical target pairing by miR-503. We identified the proto-oncogene DDHD2

SUBMITTER: Polioudakis D 

PROVIDER: S-EPMC4326481 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature