Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma.

ABSTRACT:

Background

ADAR enzymes convert adenosines to inosines within double-stranded RNAs, including microRNA (miRNA) precursors, with important consequences on miRNA retargeting and expression. ADAR2 activity is impaired in glioblastoma and its rescue has anti-tumoral effects. However, how ADAR2 activity may impact the miRNome and the progression of glioblastoma is not known.

Results

By integrating deep-sequencing and array approaches with bioinformatics analyses and molecular studies, we show that ADAR2 is essential to edit a small number of mature miRNAs and to significantly modulate the expression of about 90 miRNAs in glioblastoma cells. Specifically, the rescue of ADAR2 activity in cancer cells recovers the edited miRNA population lost in glioblastoma cell lines and tissues,

SUBMITTER: Tomaselli S 

PROVIDER: S-EPMC4326501 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature