Identification of novel radiosensitizers in a high-throughput, cell-based screen for DSB repair inhibitors.

ABSTRACT: Most cancer therapies involve a component of treatment that inflicts DNA damage in tumor cells, such as double-strand breaks (DSBs), which are considered the most serious threat to genomic integrity. Complex systems have evolved to repair these lesions, and successful DSB repair is essential for tumor cell survival after exposure to ionizing radiation (IR) and other DNA-damaging agents. As such, inhibition of DNA repair is a potentially efficacious strategy for chemo- and radiosensitization. Homologous recombination (HR) and nonhomologous end-joining (NHEJ) represent the two major pathways by which DSBs are repaired in mammalian cells. Here, we report the design and execution of a high-throughput, cell-based small molecule screen for novel DSB repair inhibitors. We miniaturized our recentl

SUBMITTER: Goglia AG 

PROVIDER: S-EPMC4326563 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature