A small intestinal organoid model of non-invasive enteric pathogen-epithelial cell interactions.

ABSTRACT: Organoids mirror in vivo tissue organization and are powerful tools to investigate the development and cell biology of the small intestine. However, their application for the study of host-pathogen interactions has been largely unexplored. We have established a model using microinjection of organoids to mimic enteric infection, allowing for direct examination of pathogen interactions with primary epithelial cells in the absence of confounding variables introduced by immune cells or the commensal microbiota. We investigated the impact of Paneth cell α-defensin antimicrobial peptides on bacterial growth. We demonstrate that organoids form a sealed lumen, which contains concentrations of α-defensins capable of restricting growth of multiple strains of Salmonella enterica serovar Typhimurium f

SUBMITTER: Wilson SS 

PROVIDER: S-EPMC4326599 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature