Cross-platform comparison of nucleic acid hybridization: toward quantitative reference standards.

ABSTRACT: Measuring interactions between biological molecules is vitally important to both basic and applied research as well as development of pharmaceuticals. Although a wide and growing range of techniques is available to measure various kinetic and thermodynamic properties of interacting biomolecules, it can be difficult to compare data across techniques of different laboratories and personnel or even across different instruments using the same technique. Here we evaluate relevant biological interactions based on complementary DNA and RNA oligonucleotides that could be used as reference standards for many experimental systems. We measured thermodynamics of duplex formation using isothermal titration calorimetry, differential scanning calorimetry, and ultraviolet-visible (UV-vis) monitored denatu

SUBMITTER: Halvorsen K 

PROVIDER: S-EPMC4326632 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature