Comparative analysis of the silk gland transcriptomes between the domestic and wild silkworms.

ABSTRACT:

Background

Bombyx mori was domesticated from the Chinese wild silkworm, Bombyx mandarina. Wild and domestic silkworms are good models in which to investigate genes related to silk protein synthesis that may be differentially expressed in silk glands, because their silk productions are very different. Here we used the mRNA deep sequencing (RNA-seq) approach to identify the differentially expressed genes (DEGs) in the transcriptomes of the median/posterior silk glands of two domestic and two wild silkworms.

Results

The results indicated that about 58% of the total genes were expressed (reads per kilo bases per million reads (RPKM) ≥ 1) in each silkworm. Comparisons of the domestic and wild silkworm transcriptomes revealed 32 DEGs, of which 16 were up-regulated in the domestic

SUBMITTER: Fang SM 

PROVIDER: S-EPMC4328555 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature