Sphingosine-1-phosphate induces pro-remodelling response in airway smooth muscle cells.

ABSTRACT:

Background

Increased proliferation of airway smooth muscle (ASM) cells leading to hyperplasia and increased ASM mass is one of the most characteristic features of airway remodelling in asthma. A bioactive lipid, sphingosine-1-phosphate (S1P), has been suggested to affect airway remodelling by stimulation of human ASM cell proliferation.

Objective

To investigate the effect of S1P on signalling and regulation of gene expression in ASM cells from healthy and asthmatic individuals.

Methods

Airway smooth muscle cells grown from bronchial biopsies of healthy and asthmatic individuals were exposed to S1P. Gene expression was analysed using microarray, real-time PCR and Western blotting. Receptor signalling and function were determined by mRNA knockdown and intracellular calc

SUBMITTER: Fuerst E 

PROVIDER: S-EPMC4329332 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature