Targeting non-coding RNAs with the CRISPR/Cas9 system in human cell lines.

ABSTRACT: The CRISPR/Cas has been recently shown to be a powerful genome-editing tool in a variety of organisms. However, these studies are mainly focused on protein-coding genes. The present study aims to determine whether this technology can be applied to non-coding genes. One of the challenges for knockout of non-coding genes is that a small deletion or insertion generated by the standard CRISPR/Cas system may not necessarily lead to functional loss of a given non-coding gene because of lacking an open reading frame, especially in polyploidy human cell lines. To overcome this challenge, we adopt a selection system that allows for marker genes to integrate into the genome through homologous recombination (HR). Moreover, we construct a dual guide RNA vector that can make two cuts simultaneously at

SUBMITTER: Ho TT 

PROVIDER: S-EPMC4330338 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature