A function-based screen for seeking RubisCO active clones from metagenomes: novel enzymes influencing RubisCO activity.

ABSTRACT: Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) is a key enzyme of the Calvin cycle, which is responsible for most of Earth's primary production. Although research on RubisCO genes and enzymes in plants, cyanobacteria and bacteria has been ongoing for years, still little is understood about its regulation and activation in bacteria. Even more so, hardly any information exists about the function of metagenomic RubisCOs and the role of the enzymes encoded on the flanking DNA owing to the lack of available function-based screens for seeking active RubisCOs from the environment. Here we present the first solely activity-based approach for identifying RubisCO active fosmid clones from a metagenomic library. We constructed a metagenomic library from hydrothermal vent fluids and screene

SUBMITTER: Bohnke S 

PROVIDER: S-EPMC4331584 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature