Integrated analyses to reconstruct microRNA-mediated regulatory networks in mouse liver using high-throughput profiling.

ABSTRACT:

Background

MicroRNAs (miRNAs) simultaneously target many transcripts through partial complementarity binding, and have emerged as a key type of post-transcriptional regulator for gene expression. How miRNA accomplishes its pleiotropic effects largely depends on its expression and its target repertoire. Previous studies discovered thousands of miRNAs and numerous miRNA target genes mainly through computation and prediction methods which produced high rates of false positive prediction. The development of Argonaute cross-linked immunoprecipitation coupled with high-throughput sequencing (CLIP-Seq) provides a system to effectively determine miRNA target genes. Likewise, the accuracy of dissecting the transcriptional regulation of miRNA genes has been greatly improved by chromatin immu

SUBMITTER: Hsu SD 

PROVIDER: S-EPMC4331712 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature