Effect of Egg White Combined with Chalcanthite on Lipopolysaccharide induced Inflammatory Cytokine Expression in RAW 264.7 cells.

ABSTRACT: AIM:Historically, mineral compound herbal medicines have long been used in treatments of immune-related diseases in Korea, China and other Asian countries. In this study, we investigated the anti-inflammatory effect of egg white combined with chalcanthite (IS4) on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. METHODS:RAW 264.7 cells cultured with LPS and various concentrations of IS4 were analyzed to determine the production of pro-inflammatory cytokines and mediators by using enzyme-linked immune sorbent assays (ELISAs). RESULTS:IS4 concentration inhibited the production of interleukin-1beta (IL-1 ?), interleukin-6 (IL-6), and granulocyte -macrophage colony-stimulating factor (GM-CSF) induced by LPS. IS4 at high concentrations (25 and 50?/ml) inhibited, in concentration-dependent manner, the expression of tumor necrosis factor-alpha (TNF- ?) stimulated by LPS. CONCLUSION:IS4 has shown an anti-inflammatory effect in RAW 264.7 cells.

SUBMITTER: Choi EA 

PROVIDER: S-EPMC4331931 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature