Involvement of Transient Receptor Potential Melastatin 7 Channels in Sophorae Radix-induced Apoptosis in Cancer Cells: Sophorae Radix and TRPM7.

ABSTRACT:

Unlabelled

Sophorae Radix (SR) plays a role in a number of physiologic and pharmacologic functions in many organs.

Objective

The aim of this study was to clarify the potential role for transient receptor potential melastatin 7 (TRPM7) channels in SR-inhibited growth and survival of AGS and MCF-7 cells, the most common human gastric and breast adenocarcinoma cell lines.

Methods

The AGS and the MCF-7 cells were treated with varying concentrations of SR. Analyses of the caspase-3 and - 9 activity, the mitochondrial depolarization and the poly (ADPribose) polymerase (PARP) cleavage were conducted to determine if AGS and MCF-7 cell death occured by apoptosis. TRPM7 channel blockers (Gd(3+) or 2-APB) and small interfering RNA (siRNA) were used in this study to confirm the r

SUBMITTER: Kim BJ 

PROVIDER: S-EPMC4331944 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature