Differential Expression of Gene Profiles in MRGX-treated Lung Cancer.

ABSTRACT:

Objectives

Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles.

Methods

To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network.

Results

One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse

SUBMITTER: Kwon YK 

PROVIDER: S-EPMC4331971 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature