Development and characterization of 47 novel microsatellite markers for Vellozia squamata (Velloziaceae).

ABSTRACT: • We developed and validated microsatellite primers for Vellozia squamata (Velloziaceae), an endemic species of the cerrado (Brazilian savannas), to investigate the influence of different fire regimes on its genetic diversity and population structure. • Using a selective hybridization method, we tested 51 SSR loci using a natural population of V. squamata and obtained 47 amplifiable loci. Among these, 26 loci were polymorphic and the average values of genetic diversity were: average number of alleles per locus ([Formula: see text]) = 6.54, average number of alleles per polymorphic locus ([Formula: see text]) = 7.13, average observed heterozygosity [Formula: see text] = 0.22, average expected heterozygosity [Formula: see text] = 0.49, and average fixation index [Formula: see text] = 0.55. •

SUBMITTER: Duarte-Barbosa M 

PROVIDER: S-EPMC4332141 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature