Defining a minimal cell: essentiality of small ORFs and ncRNAs in a genome-reduced bacterium.

ABSTRACT: Identifying all essential genomic components is critical for the assembly of minimal artificial life. In the genome-reduced bacterium Mycoplasma pneumoniae, we found that small ORFs (smORFs; < 100 residues), accounting for 10% of all ORFs, are the most frequently essential genomic components (53%), followed by conventional ORFs (49%). Essentiality of smORFs may be explained by their function as members of protein and/or DNA/RNA complexes. In larger proteins, essentiality applied to individual domains and not entire proteins, a notion we could confirm by expression of truncated domains. The fraction of essential non-coding RNAs (ncRNAs) non-overlapping with essential genes is 5% higher than of non-transcribed regions (0.9%), pointing to the important functions of the former. We found that t

SUBMITTER: Lluch-Senar M 

PROVIDER: S-EPMC4332154 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature