Bactobolin A binds to a site on the 70S ribosome distinct from previously seen antibiotics.

ABSTRACT: The ribosome is the target of a large number of antibiotics. Here, we report a 3.4-Å-resolution crystal structure of bactobolin A bound to 70S ribosome-tRNA complex. The antibiotic binds at a previously unseen site in the 50S subunit and displaces tRNA bound at the P-site. It thus likely has a similar mechanism of action as blasticidin S despite binding to a different site. The structure also rationalizes previously identified resistance mutations.

SUBMITTER: Amunts A 

PROVIDER: S-EPMC4332689 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature