Lipopeptide biosynthesis in Pseudomonas fluorescens is regulated by the protease complex ClpAP.

ABSTRACT:

Background

Lipopeptides (LP) are structurally diverse compounds with potent surfactant and broad-spectrum antibiotic activities. In Pseudomonas and other bacterial genera, LP biosynthesis is governed by large multimodular nonribosomal peptide synthetases (NRPS). To date, relatively little is known about the regulatory genetic network of LP biosynthesis.

Results

This study provides evidence that the chaperone ClpA, together with the serine protease ClpP, regulates the biosynthesis of the LP massetolide in Pseudomonas fluorescens SS101. Whole-genome transcriptome analyses of clpA and clpP mutants showed their involvement in the transcription of the NRPS genes massABC and the transcriptional regulator massAR. In addition, transcription of genes associated with cell wall and mem

SUBMITTER: Song C 

PROVIDER: S-EPMC4332742 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature