Analysis of individual mitochondria via fluorescent immunolabeling with Anti-TOM22 antibodies.

ABSTRACT: Mitochondria are responsible for maintaining a variety of cellular functions. One such function is the interaction and subsequent import of proteins into these organelles via the translocase of outer membrane (TOM) complex. Antibodies have been used to analyze the presence and function of proteins comprising this complex, but have not been used to investigate variations in the abundance of TOM complex in mitochondria. Here, we report on the feasibility of using capillary cytometry with laser-induced fluorescence to detect mitochondria labeled with antibodies targeting the TOM complex and to estimate the number of antibodies that bind to these organelles. Mitochondria were fluorescently labeled with DsRed2, while antibodies targeting the TOM22 protein, one of nine proteins comprising the TO

SUBMITTER: Taylor TH 

PROVIDER: S-EPMC4332841 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature