Modular construction of mammalian gene circuits using TALE transcriptional repressors.

ABSTRACT: An important goal of synthetic biology is the rational design and predictable implementation of synthetic gene circuits using standardized and interchangeable parts. However, engineering of complex circuits in mammalian cells is currently limited by the availability of well-characterized and orthogonal transcriptional repressors. Here, we introduce a library of 26 reversible transcription activator-like effector repressors (TALERs) that bind newly designed hybrid promoters and exert transcriptional repression through steric hindrance of key transcriptional initiation elements. We demonstrate that using the input-output transfer curves of our TALERs enables accurate prediction of the behavior of modularly assembled TALER cascade and switch circuits. We also show that TALER switches using fe

SUBMITTER: Li Y 

PROVIDER: S-EPMC4333066 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature