Direct observation of mobility state transitions in RNA trajectories by sensitive single molecule feedback tracking.

ABSTRACT: Observation and tracking of fluorescently labeled molecules and particles in living cells reveals detailed information about intracellular processes on the molecular level. Whereas light microscopic particle observation is usually limited to two-dimensional projections of short trajectory segments, we report here image-based real-time three-dimensional single particle tracking in an active feedback loop with single molecule sensitivity. We tracked particles carrying only 1-3 fluorophores deep inside living tissue with high spatio-temporal resolution. Using this approach, we succeeded to acquire trajectories containing several hundred localizations. We present statistical methods to find significant deviations from random Brownian motion in such trajectories. The analysis allowed us to dire

SUBMITTER: Spille JH 

PROVIDER: S-EPMC4333372 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature