Chromatin-dependent regulation of RNA polymerases II and III activity throughout the transcription cycle.

ABSTRACT: The particular behaviour of eukaryotic RNA polymerases along different gene regions and amongst distinct gene functional groups is not totally understood. To cast light onto the alternative active or backtracking states of RNA polymerase II, we have quantitatively mapped active RNA polymerases at a high resolution following a new biotin-based genomic run-on (BioGRO) technique. Compared with conventional profiling with chromatin immunoprecipitation, the analysis of the BioGRO profiles in Saccharomyces cerevisiae shows that RNA polymerase II has unique activity profiles at both gene ends, which are highly dependent on positioned nucleosomes. This is the first demonstration of the in vivo influence of positioned nucleosomes on transcription elongation. The particular features at the 5' end an

SUBMITTER: Jordan-Pla A 

PROVIDER: S-EPMC4333398 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature