Comparative proteomic analysis reveals activation of mucosal innate immune signaling pathways during cholera.

ABSTRACT: Vibrio cholerae O1 is a major cause of acute watery diarrhea in over 50 countries. Evidence suggests that V. cholerae O1 may activate inflammatory pathways, and a recent study of a Bangladeshi population showed that variants in innate immune genes play a role in mediating susceptibility to cholera. We analyzed human proteins present in the small intestine of patients infected with V. cholerae O1 to characterize the host response to this pathogen. We collected duodenal biopsy specimens from patients with acute cholera after stabilization and again 30 days after initial presentation. Peptides extracted from biopsy specimens were sequenced and quantified using label-free mass spectrometry and SEQUEST. Twenty-seven host proteins were differentially abundant between the acute and convalescent s

SUBMITTER: Ellis CN 

PROVIDER: S-EPMC4333457 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature