Detection of protein-small molecule binding using a self-referencing external cavity laser biosensor.

ABSTRACT: High-throughput screening has enabled the identification of small molecule modulators of important drug targets via well-established colorimetric or fluorimetric activity assays. However, existing methods to identify small molecule binders of nonenzymatic protein targets lack either the simplicity (e.g., require labeling one of the binding partners with a reporter) or throughput inherent in enzymatic assays widely used for HTS. Thus, there is intense interest in the development of high-throughput technologies for label-free detection of protein-small molecule interactions. Here we describe a novel self-referencing external cavity laser (ECL) biosensor approach that achieves high resolution and high sensitivity, while eliminating thermal noise with subpicometer wavelength accuracy. Using th

SUBMITTER: Zhang M 

PROVIDER: S-EPMC4333586 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature