Carbohydrate catabolism and the enhancement of uptake of galactose in hamster cells transformed by polyoma virus.

ABSTRACT: Untransformed as well as polyoma virus-transformed hamster cells can be grown equally well on a slow catabolite like galactose as on a rapid catabolite like glucose. The rate of uptake of galactose is greatly enhanced in the transformed cells as compared with untransformed cells, and this enhancement of entry was as markedly expressed in galactose-grown cultures as in glucose-grown cultures. Since the transformed cultures grown in glucose medium consume practically all of their carbohydrate, contrary to the galactose-grown cultures, problems dealing with regulation of transport by substrate concentrations have to be dealt with also. The galactose captured by the cells accumulates initially as galactose, alpha-galactose-1-phosphate, and UDP-galactose. However, after a 24-hr growth on a gala

SUBMITTER: Kalckar HM 

PROVIDER: S-EPMC433371 | biostudies-literature | 1973 Mar

REPOSITORIES: biostudies-literature