Transcriptome analysis of nitrogen-starvation-responsive genes in rice.

ABSTRACT:

Background

Nitrogen (N), a critical macronutrient for plant growth and development, is a major limiting factor in most agricultural systems. Microarray analyses have been conducted to investigate genome-wide gene expression in response to changes in N concentrations. Although RNA-Seq analysis can provide a more precise determination of transcript levels, it has not previously been employed to investigate the expression of N-starvation-induced genes.

Results

We constructed cDNA libraries from leaf sheaths and roots of rice plants grown under N-deficient or -sufficient conditions for 12 h. Sequencing the libraries resulted in identification of 33,782 annotated genes. A comparison of abundances revealed 1,650 transcripts that were differentially expressed (fold-change ≥ 2) due

SUBMITTER: Yang W 

PROVIDER: S-EPMC4333837 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature