Whole-genome assembly of Akkermansia muciniphila sequenced directly from human stool.

ABSTRACT:

Background

Alterations in gut microbiota composition under antibiotic pressure have been widely studied, revealing a restricted diversity of gut flora, including colonization by organisms such as Enterococci, while their impact on bacterial load is variable. High-level colonization by Akkermansia muciniphila, ranging from 39% to 84% of the total bacterial population, has been recently reported in two patients being treated with broad-spectrum antibiotics, although attempts to cultivate this microorganism have been unsuccessful.

Results

Here, we propose an original approach of genome sequencing for Akkermansia muciniphila directly from the stool sample collected from one of these patients. We performed and assembly using metagenomic data obtained from the stool sample. We use

SUBMITTER: Caputo A 

PROVIDER: S-EPMC4333879 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature