Rapid reprogramming of epigenetic and transcriptional profiles in mammalian culture systems.

ABSTRACT:

Background

The DNA methylation profiles of mammalian cell lines differ from those of the primary tissues from which they were derived, exhibiting increasing divergence from the in vivo methylation profile with extended time in culture. Few studies have directly examined the initial epigenetic and transcriptional consequences of adaptation of primary mammalian cells to culture, and the potential mechanisms through which this epigenetic dysregulation occurs is unknown.

Results

We demonstrate that adaptation of mouse embryonic fibroblasts to cell culture results in a rapid reprogramming of epigenetic and transcriptional states. We observed global 5-hydroxymethylcytosine (5hmC) erasure within three days of culture initiation. Loss of genic 5hmC was independent of global 5-methyl

SUBMITTER: Nestor CE 

PROVIDER: S-EPMC4334405 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature