Mechanical preconditioning enables electrophysiologic coupling of skeletal myoblast cells to myocardium.

ABSTRACT:

Objective

The effect of mechanical preconditioning on skeletal myoblasts in engineered tissue constructs was investigated to resolve issues associated with conduction block between skeletal myoblast cells and cardiomyocytes.

Methods

Murine skeletal myoblasts were used to generate engineered tissue constructs with or without application of mechanical strain. After in vitro myotube formation, engineered tissue constructs were co-cultured for 6 days with viable embryonic heart slices. With the use of sharp electrodes, electrical coupling between engineered tissue constructs and embryonic heart slices was assessed in the presence or absence of pharmacologic agents.

Results

The isolation and expansion procedure for skeletal myoblasts resulted in high yields of homogeneousl

SUBMITTER: Neef K 

PROVIDER: S-EPMC4334564 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature