Control of the initiation and termination of kinesin-1-driven transport by myosin-Ic and nonmuscle tropomyosin.

ABSTRACT: Intracellular transport is largely driven by processive microtubule- and actin-based molecular motors. Nonprocessive motors have also been localized to trafficking cargos, but their roles are not well understood. Myosin-Ic (Myo1c), a nonprocessive actin motor, functions in a variety of exocytic events, although the underlying mechanisms are not yet clear. To investigate the interplay between myosin-I and the canonical long-distance transport motor kinesin-1, we attached both motor types to lipid membrane-coated bead cargo, using an attachment strategy that allows motors to actively reorganize within the membrane in response to the local cytoskeletal environment. We compared the motility of kinesin-1-driven cargos in the absence and presence of Myo1c at engineered actin/microtubule intersec

SUBMITTER: McIntosh BB 

PROVIDER: S-EPMC4334669 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature