Targeted deep sequencing reveals no definitive evidence for somatic mosaicism in atrial fibrillation.

ABSTRACT:

Background

Studies of ≤15 atrial fibrillation (AF) patients have identified atrial-specific mutations within connexin genes, suggesting that somatic mutations may account for sporadic cases of the arrhythmia. We sought to identify atrial somatic mutations among patients with and without AF using targeted deep next-generation sequencing of 560 genes, including genetic culprits implicated in AF, the Mendelian cardiomyopathies and channelopathies, and all ion channels within the genome.

Methods and results

Targeted gene capture and next-generation sequencing were performed on DNA from lymphocytes and left atrial appendages of 34 patients (25 with AF). Twenty AF patients had undergone cardiac surgery exclusively for pulmonary vein isolation and 17 had no structural heart disease

SUBMITTER: Roberts JD 

PROVIDER: S-EPMC4334693 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature