Models for predicting effective HIV chemoprevention in women.

ABSTRACT:

Objective

Model systems that rapidly identify tissue drug concentrations protective of HIV infection could streamline the development of chemoprevention strategies. Tissue models are promising, but limited concentration targets exist, and no systematic comparison to cell models or clinical studies has been performed.

Design

We explored the efficacy of maraviroc (MVC) and tenofovir (TFV) for HIV prevention by comparing Emax models from TZM-bl cells to vaginal tissue explants and evaluated their predictive capabilities with a dose-challenge clinical study.

Methods

HIV-1JR-CSF was used for viral challenge. Drug efficacy was assessed using a luciferase reporter assay in TZM-bl cells and real-time PCR to quantify spliced RNA in a tissue explant model. Cell and tissue conce

SUBMITTER: Nicol MR 

PROVIDER: S-EPMC4334725 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature