Epithelial-mesenchymal transitioned circulating tumor cells capture for detecting tumor progression.

ABSTRACT:

Purpose

This study aimed to detect cell-surface vimentin (CSV) on the surface of epithelial-mesenchymal transitioned (EMT) circulating tumor cells (CTC) from blood of patients with epithelial cancers.

Experimental design

In this study, 101 patients undergoing postsurgery adjuvant chemotherapy for metastatic colon cancer were recruited. EMT CTCs were detected from blood of patients using the 84-1 monoclonal antibody against CSV as a marker. EMT CTCs isolated were characterized further using EMT-specific markers, fluorescent in situ hybridization, and single-cell mutation analysis.

Results

Using the 84-1 antibody, we detected CSV exclusively on EMT CTCs from a variety of tumor types but not in the surrounding normal cells in the blood. The antibody exhibited very high s

SUBMITTER: Satelli A 

PROVIDER: S-EPMC4334736 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature