Pluripotent stem cell miRNAs and metastasis in invasive breast cancer.

ABSTRACT:

Background

The purpose of this study is to determine whether microRNA for pluripotent stem cells are also expressed in breast cancer and are associated with metastasis and outcome.

Methods

We studied global microRNA profiles during differentiation of human embryonic stem cells (n =26) and in breast cancer patients (n = 33) and human cell lines (n = 35). Using in situ hybridization, we then investigated MIR302 expression in 318 untreated breast cancer patients (test cohort, n = 22 and validation cohort, n = 296). In parallel, using next-generation sequencing data from breast cancer patients (n = 684), we assessed microRNA association with stem cell markers. All statistical tests were two-sided.

Results

In healthy tissues, the MIR302 (high)/MIR203 (low) asymmetry was ex

SUBMITTER: Volinia S 

PROVIDER: S-EPMC4334797 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature